Reagent kit
qPCR · triplex · DIVA
Bio-T kit® BHV1 DIVA, PCR
On backorder
SKU BIOTK098
50 tests
- Method
- PCR/qPCR · Polymerase chain reaction, including real-time PCR
- Test type
- Pathogen detection (DNA/RNA)
- Species
- Cattle
Price on request
Individual pricing for your profile — provided in reply to your request.
Overview
A ready-to-use kit for the detection of all Bovine Herpesvirus type 1 strains (BHV1 gB) and differentiation of gE-deleted vaccine strains (BHV1 gE) by real-time PCR, for the diagnosis of infectious bovine rhinotracheitis. Contains an endogenous internal positive control (IPC).
- Developed in compliance with U47-600-2 (AFNOR)
- DIVA: distinguishes field infection from gE-deleted vaccine
- Ready-to-use blue Master Mix — thaws quickly and is easy to identify
- Reliable BHV1 diagnosis thanks to an endogenous IPC — high-quality results on all matrices
- Made in France
| Method | Real-time PCR (qPCR), triplex, DIVA |
|---|---|
| Target | Bovine herpesvirus type 1 (BHV1): gB — all strains, gE — vaccine differentiation |
| Sample types | bronchoalveolar lavage, trans-tracheal aspirate, deep nasopharyngeal swab, cervical swab, lung, sheath wash (semen), fetal organs. Individually or in pools of up to 3. |
| Internal control | Endogenous internal positive control (IPC) |
| Detection channels | 6-FAM, VIC, Cy5 |
| Instruments | ABI PRISM® 7500 Fast (Applied Biosystems), AriaMx™ (Agilent); compatible with any thermal cycler with the required detection channels |
| Pack size | 50 tests |
| Manufacturer | Biosellal, France |