Method Reverse-transcription real-time PCR (qRT-PCR), duplex
Target Classical swine fever virus (CSFV)
Sample types Whole blood (EDTA), serum, plasma, cell culture supernatant, organs (spleen, tonsil, lymph node), swabs (blood or exudate). Individual analyses.
Internal control Endogenous internal positive control (IPC)
Validation French CSF/ASF NRL (ANSES)
Detection channels 6-FAM, Cy5
Instruments AriaMx™ (Agilent), ABI PRISM® 7500 Fast, Rotor-Gene Q (QIAGEN)
Pack size 50 tests / 100 tests
Manufacturer Biosellal, France

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