Reagent kit
qPCR · duplex
Bio-T kit® Lumpy Skin Disease, PCR
On backorder
SKU BIOTK034
50 tests
- Method
- PCR/qPCR · Polymerase chain reaction, including real-time PCR
- Test type
- Pathogen detection (DNA/RNA)
- Species
- Cattle
Price on request
Individual pricing for your profile — provided in reply to your request.
Overview
A ready-to-use kit for the detection of Lumpy Skin Disease virus (LSDV) by real-time PCR, for the diagnosis of Lumpy Skin Disease in cattle. Contains an endogenous internal positive control (IPC). Evaluated by the Pirbright Institute (UK), the WOAH reference laboratory for capripoxviruses, and validated by CIRAD (France).
- Developed in accordance with U47-600-2 (AFNOR), validated by CIRAD (France) and evaluated by the Pirbright Institute (UK)
- Ready-to-use blue Master Mix — thaws quickly and is easy to identify
- Reliable LSDV detection thanks to an endogenous IPC — high-quality results on all matrices, even the most difficult ones
- Extraction and amplification programme shared with all ruminant kits
- Made in France
| Method | Real-time PCR (qPCR), duplex |
|---|---|
| Target | Lumpy Skin Disease virus (LSDV) |
| Sample types | Whole blood (EDTA), dry oral, nasal and conjunctival swabs, skin lesion swabs and scrapings, closed nodule biopsies. Individual analyses. |
| Internal control | Endogenous internal positive control (IPC) |
| Validation | CIRAD (France); evaluated by the Pirbright Institute (UK) |
| Detection channels | 6-FAM, Cy5 |
| Instruments | ABI PRISM® 7500 Fast (Applied Biosystems), AriaMx™ (Agilent); compatible with any thermal cycler with the required detection channels |
| Pack size | 50 tests |
| Manufacturer | Biosellal, France |