Target African swine fever virus (ASFV) DNA
Method Loop-mediated isothermal amplification (LAMP)
Pack size 96 reactions (single tubes)
Protocol 25 min at 65 °C, SYBR/FAM channel
Reaction volume 25 µL: 20 µL LAMP buffer + 5 µL sample
Confirmation Melt curve, Tm 87.0 ± 0.5 °C
Validated on Genie® Lite (OptiGene)
Storage Shipped at room temperature; then −15 to −25 °C, 1 year
Not included Nucleic acid extraction kit, micropipettes with filter tips, vortex, mini centrifuge, cooling block
Status Research Use Only
Manufacturer Genetic PCR Solutions®, Spain

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