Purpose On-farm detection of African swine fever virus (ASFV)
Method Loop-mediated isothermal amplification (LAMP)
Time to result Under 30 minutes (reaction — 25 min at 65 °C)
Step 1 — sampling GPSponge® kit
Step 2 — reaction ASFV MONODOSE-LAMP® tubes, 24 or 96 tests
Step 3 — analysis Genie® Lite portable fluorometer
Confirmation Melt curve, Tm 87.0 ± 0.5 °C
Validation Developed and validated with IRTA-CReSA
Reagent status Research Use Only
Manufacturers Genetic PCR Solutions® (Spain), OptiGene Ltd (UK)

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